Autophagy，the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR （Target of rapamycin） through phosphorylation of autophagy protein APG1. Another member of the autophagy protein family is APG10 （Also called ATG10，APG10L），an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and the modification of a soluble form of MAP-LC3，a homolog of yeast Apg8，to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Multiple isoforms of ATG10 are known to exist.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Other applications have not been determined.
免疫原
Synthetic peptide， near the carboxyl terminus of human APG10
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的APG10/ATG10蛋白發生免疫反應.
保存建議
提供的APG10/ATG10抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5 mg/ml BSA和0.01%硫柳汞nn的PBS緩沖液中.請將該APG10/ATG10抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
