Autophagy，the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR （Target of rapamycin） through phosphorylation of autophagy protein APG1. ATG12，another member of the autophagy protein family，forms a conjugate with ATG5； this conjugate has a ubiquitin-protein ligase （E3）-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA （also known as IPS-1），inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 21 of mouse APG12
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的APG12/ATG12蛋白發生免疫反應.
保存建議
提供的APG12/ATG12抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5 mg/ml BSA和0.01%硫柳汞的PBS緩沖液中.請將該APG12/ATG12抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
