Autophagy，the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR （Target of rapamycin） through phosphorylation of autophagy protein APG1. ATG16，another member of the autophagy protein family，forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain，it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Other applications have not been determined.
免疫原
Synthetic peptide surrounding amino acid 565 of human APG16
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的APG16/ATG16蛋白發生免疫反應.
保存建議
提供的APG16/ATG16抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5 mg/ml BSA和0.01%硫柳汞的PBS緩沖液中.請將該APG16/ATG16抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
