Polycomb group （PcG） genes encode chromatin proteins that are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Bmi-1 has been identified among the PcG genes as a potent oncogene that plays an important role in the regulation of cell proliferation by suppressing the p16-dependant pathway. Bmi-1 may be use as a therapeutic target for studying stem cell proliferation and renewal.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes ~34 kDa of Bmi-1 in Jurkat cell lysate and rat kidney tissue lysate. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 312 of rat BMI-1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、牛、狗、cat種屬來源的BMI-1蛋白發生免疫反應.
保存建議
提供的BMI-1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該BMI-1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
