Methylation of DNA at cytosine residues plays an important role in regulation of gene expression，genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date，3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1，Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality.
應用類型
Western blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Rat kidney tissue lysate can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid 1208 of rat DNMT1.
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的DNMT1蛋白發生免疫反應.
保存建議
提供的DNMT1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該DNMT1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
