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細胞分析試劑盒
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中文名稱

BMP-7 ELISA Kit (Rat)

英文名字
BMP-7 ELISA Kit (Rat)
供應商
USBiological
產品貨號
396589
產品報價
¥1/96Tests
產品說明書
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購買方式
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背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-Beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.Sample Type:Cell culture supernates, serum, tissue, bone tissue, plasma (EDTA or Heparin).Intended Use:Sandwich High Sensitivity BioAssay ELISA kit for Quantitative Detection of Rat BMP-7.Sensitivity:<10pg/mlRange:31.2pg/ml-2000pg/mlSpecificity:Recognizes natural and recombinant Rat BMP-7. There is no detectable cross-reactivity with other relevant proteins.Test Principle:This Rat BMP-7 BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for BMP-7 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO; Immunogen sequence: S292-H430) and test samples are added to the wells, a biotinylated detection specific for BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat BMP-7 amount of sample captured in plate.Kit Components:96-well plate precoated with anti-Rat Bmp7 antibody, 1x plateLyophilized recombinant Rat Bmp7 standard, 2x10ng/tubeBiotinylated anti-Rat Bmp7 antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x bufferStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產品特點
針對BMP-7 ELISA Kit (Rat)該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到BMP-7 ELISA Kit (Rat)產品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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