- 中文名稱
E-Cadherin BioAssay ELISA Kit (Rat)
- 英文名字
- E-Cadherin BioAssay ELISA Kit (Rat)
- 供應商
- USBiological
- 產品貨號
- 396634
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- Cadherin-1, also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin, is a protein that in humans is encoded by the CDH1 gene. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E- cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. It plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120kD.Sample Type:Cell culture supernates, serum, plasma (Heparin)Intended Use:Sandwich High Sensitivity BioAssay ELISA kit for Quantitative Detection of Rat E-Cadherin.Sensitivity:<15pg/mlRange:156pg/ml-10,000pg/mlSpecificity:Recognizes natural and recombinant Rat E-Cadherin. There is no detectable cross-reactivity with other relevant proteins.Test Principle:This Rat E-Cadherin BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for E-Cadherin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: Q24-S704) and test samples are added to the wells, a biotinylated detection specific for E-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat E-Cadherin amount of sample captured in plate.Kit Components:96-well plate precoated with anti-Rat Cdh1 antibody, 1x plateLyophilized recombinant Rat Cdh1 standard, 2x10ng/tubeBiotinylated anti-Rat Cdh1 antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x bufferStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對E-Cadherin BioAssay ELISA Kit (Rat)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到E-Cadherin BioAssay ELISA Kit (Rat)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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