The T7-tag is an 11 amino acid sequence encoded in the leader sequence of T7 bacteriophage gene10. T7 is used in many expression vectors including the pET system. Hence,the anti-T7-tag antibody is useful for studying expression of recombinant T7-tagged proteins.
應用類型
The antibody can be used for Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually.
免疫原
A synthetic T7-Tag peptide MASMTGGQQMG conjugated to KLH.
來源宿主
兔
反應性
經檢測驗證,可與T7-tag標簽及T7-tag融合蛋白種屬來源的T7標簽蛋白發生免疫反應
保存建議
提供的100 ug T7 Epitope -Tag抗體產品為無色溶液其濃度為0.5mg/ml溶解于包含有30%甘油, 0.5% BSA, 5 mM EDTA和0.01%硫柳汞的PBS (pH 7.2)緩沖液中.請將該T7 Epitope -Tag抗體產品置于-20 °C冷凍保存,建議分裝使用以避免反復凍融.