DYKDDDDK Tag antibody detects over-expressed proteins containing the D epitope tag fused to either the amino- or carboxy-terminus of targeted proteins. Epitope tags are usually for the labeling and detection of proteins using immunoblotting，immunoprecipitation and immunostaining techniques. Due to their small size，they are unlikely to affect the tagged protein’s biochemical properties. The DYKDDDK epitope tag is widely used to detect expression of recombinant proteins in bacteria，yeast，insect and mammalian systems
應用類型
The antibody can be used for Western blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
A synthetic D- tag peptide DYKDDDDK conjugated to KLH.
來源宿主
兔
反應性
經檢測驗證，可與D-tag標簽及D-tag融合蛋白種屬來源的D-Tag標簽蛋白發生免疫反應
保存建議
提供的100 ug D-Tag抗體產品為無色溶液其濃度為0.5mg/ml溶解于包含有30%甘油， 0.5% BSA， 5 mM EDTA和0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該D-Tag抗體產品置于-20 °C冷凍保存，建議分裝使用以避免反復凍融.
