Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene，GFP has become a powerful tool with exciting applications in developmental，cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria，yeast，plant and mammalian cells. GFP can also fuse with proteins of interest without interfering significantly with their assembly and function.
應用類型
Western blotting （0.5-4 ug/ml）， immunoprecipitation （2-8 ug/ml）， immunofluorescence （10-20 ug/ml） and Immunohistochemistry （10-20 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes wild-type GFP and its derivatives such as EGFP， EBFP， ECFP， and EYFP， etc. Performs well in Western blot analysis （0.5 to 4 ug/ml）， immunoprecipitation， and Immunohistochemistry. However， the optimum conditions should be determined individually.
免疫原
Recombinant EGFP expressed from E. col
來源宿主
小鼠
反應性
經檢測驗證，可與所有種屬來源的GFP蛋白發生免疫反應.
保存建議
提供的GFP抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該GFP抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
