Epitope tags are usually for the labeling and detection of proteins using immunoblotting，immunoprecipitation and immunostaining techniques. Due to their small size，they are unlikely to affect the tagged protein’s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria，yeast，insect and mammalian systems.
應用類型
Western blotting （1-2 ug/ml）， immunoprecipitation （20-40 ug/ml）， and immunocytochemistry （10-20 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects Myc-Tag proteins overexpressed in cells. It does not detect endogenous levels of the transcription factor c-Myc.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 413 of human c-Myc
來源宿主
兔
反應性
經檢測驗證，可與所有種屬來源的Myc-Tag蛋白發生免疫反應.
保存建議
提供的Myc-Tag抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS， pH 7.2），緩沖液中.請將該Myc-Tag抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
