A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine （His） residues，followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags，this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
應用類型
Western blotting （1 ug/ml）， immunoprecipitation （40 ug/ml）， and immunocytochemistry （2 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects His-tagged recombinant proteins.
免疫原
6xHis synthetic peptide
來源宿主
兔
反應性
經檢測驗證，可與所有種屬來源的His-Tag蛋白發生免疫反應.
保存建議
提供的His-Tag抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS， pH 7.2），緩沖液中.請將該His-Tag抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
