Green fluorescent protein （GFP） is a spontaneously fluorescent protein isolated from pacific jellyfish，Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene，GFP has become a powerful tool with exciting applications in developmental，cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria，yeast，plant and mammalian cells. GFP can fuse with proteins of interest without interfering significantly with their assembly and function.
應用類型
Western blotting （0.5-4 ug/ml） and in immunoprecipitation （10-20 ug/ml）. However， the optimal conditions should be determined individually. The antibody reacts with GFP， and its variants EGFP， RFP， YFP， and CFP， etc. Recombinant EGFP （Cat.# 4999-100）， RFP （Cat.# 4997-100）， YFP （Cat.# 4998-100） and CFP （Cat.# 4996-100） can be used as positive controls.
免疫原
Recombinant EGFP
來源宿主
兔
反應性
經檢測驗證，可與所有種屬來源的GFP蛋白發生免疫反應.
保存建議
提供的GFP抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該GFP抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
