Digoxigenin is a cardenolide steroid isolated from the plant species of the genus Digitalis. It has a role as a hapten and a plant metabolite and can be obtained by hydrolysis of digoxin. Digoxigenin or DIG is one of the most common reporters used with nucleotides and requires the use of a conjugated anti-digoxigenin antibody. Since digoxigenin is a plant-related chemical not found in mammalian tissues, it is widely used as a method for generating non-radioactive probes, and is an alternative to biotin. DIG is an all-purpose immuno-tag, and in particular a standard marker for immunohistochemistry and in situ hybridization that supports both chromogenic and chemiluminescence detection.nnA single-domain antibody (sdAb) is a small antibody fragment consisting of the monomeric variable domain derived from camelid heavy chain-only immunoglobulins naturally found in llamas, alpacas and camels. Also known as VHH antibodies, these are the smallest functional antigen-binding fragment that occurs in nature (12 - 14 kDa) and are now being used in biotechnology as a novel antibody scaffold. The small size of the VHH single domain antibody makes it very attractive for use in diagnostic imaging and potentially therapeutic applications.
應用類型
ELISA,Western Blot,
免疫原
該【干冰】Anti-DIG (VHH) Single Domain Antibody - 400-001-MG3的詳細信息查看Rockland提供的產品說明書。
來源宿主
該【干冰】Anti-DIG (VHH) Single Domain Antibody - 400-001-MG3的詳細信息查看Rockland提供的產品說明書。
反應性
Digitalis sp.
保存建議
Store at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
