The mammalian Matrix metalloproteinases （MMPs） degrade extracellular matrix in physiological and pathological processes. After cleavage of a single peptide domain of about 20 amino acids，the MMPs are secreted in latent forms. Upon activation，the N-terminal propeptide domain is cleaved to generate the active forms of MMP. MMP-2 （72 kDa type IV collagenase，Gelatinase-A） contains the basic structure of propeptide，catalytic，and hemopexin domains. It is an important proteinase in tissue remodeling.
應用類型
Western blot analysis （0.5-4 ug/ml） and immunoprecipitation （4-8 ug/ml）. However， the optimal conditions should be determined individually. The antibody has no cross-reactivity with other MMP family members. Cross-reactivity to MMP-2 of other species has not been determined.
免疫原
E. coli-expressed active rat 72-kDa type IV collagenase
來源宿主
兔
反應性
經檢測驗證，可與大鼠種屬來源的MMP-2蛋白發生免疫反應.
保存建議
提供的MMP-2抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該MMP-2抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
