MIF （macrophage migration inhibitory factor） was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of microphages. Recently，MIF was rediscovered as a pituitary hormone that acts as the counter-regulatory hormone for glucocorticoid action within the immune system. MIF was released from macrophages and T-cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. MIF also plays a critical role in the host control of inflammation and immunity.
應用類型
Western blotting （0.5-4 ug/ml） and immunoprecipitation （5-10 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 67 of human MIF
來源宿主
兔
反應性
經檢測驗證，可與小鼠、大鼠種屬來源的MIF蛋白發生免疫反應.
保存建議
提供的MIF抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該MIF抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
