EGF-D is most closely related to VEGF-C by virtue of the presence of N- and C-terminal extensions that are not found in other VEGF family members. In adult human tissues，VEGF-D mRNA is most abundant in heart，lung，skeletal muscle，colon，and small intestine. Analyses of VEGF-D receptor specificity revealed that VEGF-D is a ligand for both VEGF receptors （VEGFRs） VEGFR-2 （Flk1） and VEGFR-3 （Flt4） and can activate these receptors. However，VEGF-D does not bind to VEGFR-1. Expression of a truncated derivative of VEGF-D demonstrated that the receptor-binding capacities reside in the portion of the molecule that is most closely related in primary structure to other VEGF family members and that corresponds to the mature form of VEGF-C. In addition，VEGF-D is a mitogen for endothelial cells. The recombinant VEGF-D as the immunogen was expressed based on sequence from Genebank Accession# D89630，20-354 aa.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Recombinant human VEGF-D （Cat.# 4643-10） can be used as a positive control. Other applications have not been determined.
免疫原
E. coli expressed human VEGF-D
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的VEGF-D蛋白發生免疫反應.
保存建議
提供的VEGF-D抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該VEGF-D抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
