ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. ApoE is an important constituent of all plasma lipoproteins. It’s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells，which is an essential step during normal lipid metabolism. It also binds with the LDL receptor （apo B/E）. Defects in ApoE are a cause of hyperlipoproteinemia type III. ApoE exists in three major isoforms； E2，E3，and E4，which differ from one another by a single amino-acid substitution. E3 is the most common isoform and is present in 40-90% of the population.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. Recombinant ApoA1 （Cat. # 4696-100） can be used as a positive control. The antibody recognizes ~34 kDa of human ApoE3. Reactivity to other species has not been tested.
免疫原
Recombinant human ApoE3
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的ApoE3蛋白發生免疫反應.
保存建議
提供的ApoE3抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該ApoE3抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
