ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids，these proteins help to maintain the structural integrity of lipoproteins，serve as ligands for lipoprotein receptors，and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. It’s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells，which is an essential step during normal lipid metabolism. It also binds with the LDL receptor （apo B/E）. Defects in ApoE are a cause of hyperlipoproteinemia type III. ApoE exists in three major isoforms； E2，E3，and E4，which differ from one another by a single amino-acid substitution. Individuals heterozygous for the ApoE4 allele are at higher risk of late-onset Alzheimer’s disease.
應用類型
Western blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Recombinant human ApoE4 （Cat.# 4699-50） can be used as a positive control.
免疫原
Recombinant human ApoE4
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的ApoE4蛋白發生免疫反應.
保存建議
提供的ApoE4抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞nn的PBS （pH 7.2）緩沖液中.請將該ApoE4抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
