Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization，and resulting accumulation，of the intracellular signaling protein，beta-catenine. Wnt signaling induces and maintains the transformed phenotype and，in certain embryonic cell lines，supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues.
應用類型
Western blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Recombinant human Wnt-1 （Cat.# 4754-10） can be used as a positive control.
免疫原
E. coli expressed human Wnt-1
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的Wnt-1蛋白發生免疫反應.
保存建議
提供的Wnt-1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Wnt-1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
