The recombinant CFP (Cyan Fluorescent Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal CFP fluorescence. The protein is suitable as a control reagent for CFP expression studies or as a labeling reagent. Applications include: Use as standards for SDS-PAGE,Western blot analysis of CFP transfected cells,label other proteins,calibration of fluorometers and flow cytometers,fluorescence microscope,or microinjection of CFP into cells and tissues,etc.
應用類型
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Recombinant CFP (Cat.# 4996-100) can be used a positive control.
免疫原
E. coli expressed CFP
來源宿主
兔
反應性
經檢測驗證,可與所有種屬來源的CFP蛋白發生免疫反應.
保存建議
提供的CFP抗體產品為100 ug 無色溶液,其濃度為0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS (pH 7.2)緩沖液中.請將該CFP抗體產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.