Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. The emission spectra for this DyLight? conjugate match the principle output wavelengths of most common fluorescence instrumentation. Dylight? antibody and reagent conjugates are specifically designed for protein detection methods that cover much of the visible spectrum and into the near-infrared region to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. Dylight?conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging.
應用類型
FLISA,IF Microscopy,Western Blot,
免疫原
Fluorescein conjugated to Goat IgG
來源宿主
Goat
反應性
該Anti-FLUORESCEIN (GOAT) Antibody DyLight? 405 Conjugated - 600-146-096的詳細信息查看Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. The emission spectra for this DyLight? conjugate match the principle output wavelengths of most common fluorescence instrumentation. Dylight? antibody and reagent conjugates are specifically designed for protein detection methods that cover much of the visible spectrum and into the near-infrared region to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. Dylight?conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging.提供的產品說明書。
保存建議
Store vial at 4° C prior to restoration.?? For extended storage aliquot contents and freeze at -20° C or below.? Avoid cycles of freezing and thawing.? Centrifuge product if not completely clear after standing at room temperature.? This product is stable for several weeks at 4° C as an undiluted liquid.? Dilute only prior to immediate use.
