Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure.? Disruption of apoptosis may contribute to cancer and other diseases.? Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. Caspase-2 has been called ICH-1 in human, CED-3 in C.elegans and NEDD-2 in mouse.? Caspase-2 is synthesized as an inactive precursor that is processed in cells undergoing apoptosis.? The precursor form of all caspases is composed of a pro-domain, and large and small catalytic subunits.? The active forms of caspases are generated by several stimuli, including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions.? Caspase-2 is activated in vitro by caspase-1, caspase-3 and granzyme B.? Caspase-2 functions as an upstream apoptosis initiator that participates in the activation of downstream caspases, such as caspase-3, caspase-6 and caspase-7.? Caspase-2 is highly expressed in embryonic mouse brain but not in adult neural tissue.? Alternative mRNA splicing produces a long (48 kDa) and short (35 kDa) form of caspase-2.? These forms show tissue specificity and function either as inducers or suppressors of apoptosis.? Mature caspase-2 is 435 amino acids in length cleaved (~50 kDa) and is cleaved upon activation into 10 and 20 kDa subunits.
應用類型
ELISA,Western Blot,
免疫原
A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.
來源宿主
Rabbit
反應性
Mus musculus (Mouse); Rattus (Rat)
保存建議
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
