- 中文名稱
【干冰】Anti-Yeast Rad9 pS1260 (RABBIT) Antibody - 600-401-468
- 英文名字
- 【干冰】Anti-Yeast Rad9 pS1260 (RABBIT) Antibody - 600-401-468
- 供應商
- Rockland
- 產品貨號
- 600-401-468
- 產品報價
- ￥詢價/100ug
- 產品說明書
- 點擊查看
- 購買方式
- 90%產品中國有現貨庫存。銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- Rad9 is required for the MEC1/TEL1-dependent activation of Saccharomyces cerevisiae DNA damage checkpoint pathways mediated by Rad53 and Chk1. DNA damage induces Rad9 phosphorylation, and Rad53 specifically associates with phosphorylated Rad9.? Cells have evolved multiple strategies for tolerating genomic damage. The most important of these are numerous repair systems that remove or bypass potentially mutagenic DNA lesions.? Another cellular strategy is to delay cell-cycle transitions at multiple points.? The genetic control of these delays, termed `checkpoints', was first established in budding yeast where it was shown that the RAD9 gene functions in G2/M arrest after irradiation with X-rays.? Subsequently, it has become clear that Rad9 also functions at the G1/S, intra-S and mid-anaphase checkpoints. ?Defects in checkpoint regulation can lead to genome instability and, in higher eukaryotes, neoplastic transformation. Rad9 also controls the transcriptional induction of a DNA damage regulon (DDR).? Rad9 may also have a pro-apoptotic function.? This is suggested in that Rad9 from Schizosaccharomyces pombe (SpRad9) contains a group of amino acids with similarity to the Bcl-2 homology 3 death domain, which is required for SpRad9 interaction with human Bcl-2 and apoptosis induction in human cells.? Overexpression of Bcl-2 in S. pombe inhibits cell growth independently of rad9, but enhances resistance of rad9-null cells to methyl methanesulfonate, ultraviolet and ionizing radiation. Rad9 conveys the checkpoint signal by activating Rad53p and Chk1p; is hyperphos-phorylated by Mec1p and Tel1p; and is a potential Cdc28p substrate. Mature yeast Rad9 is reported to have an apparent molecular weight of ~148kDa.? The human homolog is reported at 48.5 kDa.
- 應用類型
- ELISA,Western Blot,
- 免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to phosphorylated form of an internal region near aa 1225-1275 from the aa1309 yeast Rad9 protein conjugated to KLH.
- 來源宿主
- Rabbit
- 反應性
- 該【干冰】Anti-Yeast Rad9 pS1260 (RABBIT) Antibody - 600-401-468的詳細信息查看Rad9 is required for the MEC1/TEL1-dependent activation of Saccharomyces cerevisiae DNA damage checkpoint pathways mediated by Rad53 and Chk1. DNA damage induces Rad9 phosphorylation, and Rad53 specifically associates with phosphorylated Rad9.? Cells have evolved multiple strategies for tolerating genomic damage. The most important of these are numerous repair systems that remove or bypass potentially mutagenic DNA lesions.? Another cellular strategy is to delay cell-cycle transitions at multiple points.? The genetic control of these delays, termed `checkpoints', was first established in budding yeast where it was shown that the RAD9 gene functions in G2/M arrest after irradiation with X-rays.? Subsequently, it has become clear that Rad9 also functions at the G1/S, intra-S and mid-anaphase checkpoints. ?Defects in checkpoint regulation can lead to genome instability and, in higher eukaryotes, neoplastic transformation. Rad9 also controls the transcriptional induction of a DNA damage regulon (DDR).? Rad9 may also have a pro-apoptotic function.? This is suggested in that Rad9 from Schizosaccharomyces pombe (SpRad9) contains a group of amino acids with similarity to the Bcl-2 homology 3 death domain, which is required for SpRad9 interaction with human Bcl-2 and apoptosis induction in human cells.? Overexpression of Bcl-2 in S. pombe inhibits cell growth independently of rad9, but enhances resistance of rad9-null cells to methyl methanesulfonate, ultraviolet and ionizing radiation. Rad9 conveys the checkpoint signal by activating Rad53p and Chk1p; is hyperphos-phorylated by Mec1p and Tel1p; and is a potential Cdc28p substrate. Mature yeast Rad9 is reported to have an apparent molecular weight of ~148kDa.? The human homolog is reported at 48.5 kDa.提供的產品說明書。
- 保存建議
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 其他
- Rockland成立于1962年，是一家老牌抗體原產企業，其位于美國費城郊區的農場保有近20種實驗動物。Rockland公司為生命科學領域提供豐富的、高質量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質、轉錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應用十分廣泛。
- 注意
-
該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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