【干冰】Anti-MLF1 Interacting Protein (internal) (RABBIT) Antibody - 600-401-923-【干冰】Anti-MLF1 Interacting Protein (internal) (RABBIT) Antibody

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中文名稱: 【干冰】Anti-MLF1 Interacting Protein (internal) (RABBIT) Antibody - 600-401-923

英文名字: 【干冰】Anti-MLF1 Interacting Protein (internal) (RABBIT) Antibody - 600-401-923

背景資料: This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate.? Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition.? Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry.? Mutations of these sites stabilize MLF1IP and inhibit mitotic progression.? Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation.? Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block.? Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset.? MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation.? Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

免疫原: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein.

反應性: Bovine (Bos Taurus); Canine (Dog); H. sapiens (Human); Pan troglodytes (Chimpanzee)

保存建議: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

其他: Rockland成立于1962年，是一家老牌抗體原產企業，其位于美國費城郊區的農場保有近20種實驗動物。Rockland公司為生命科學領域提供豐富的、高質量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質、轉錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應用十分廣泛。