Apoptosis is related to development as well as to many diseases.? The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. ?A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3).? PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members.? This family of proteins also includes Bax and Noxa, which are also transcriptional targets of p53. ?The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53.? PUMA may be a direct mediator of p53-induced apoptosis. Anti-PUMA antibody is ideal for investigators involved in Apoptosis research.
應用類型
ELISA,Western Blot,
免疫原
PUMA Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to amino acids near the c-terminus of human PUMA-a. This sequence is identical between a and b forms of the PUMA proteins.
來源宿主
Rabbit
反應性
H. sapiens (Human); Mus musculus (Mouse)
保存建議
Store vial at 2° - 8° C or below prior to opening. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening product.
