Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.
應用類型
FLISA,IF Microscopy,Western Blot,
免疫原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 114-122 (Y-P-Y-D-V-P-D-Y-A-G) of hemagglutinin influenza conjugated to KLH using maleimide.
來源宿主
Rabbit
反應性
該Anti-HA EPITOPE TAG (RABBIT) Antibody DyLight? 680 Conjugated - 600-444-384的詳細信息查看Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.提供的產品說明書。
保存建議
Store vial at 4° C prior to restoration.?? For extended storage aliquot contents and freeze at -20° C or below.? Avoid cycles of freezing and thawing.? Centrifuge product if not completely clear after standing at room temperature.? This product is stable for several weeks at 4° C as an undiluted liquid.? Dilute only prior to immediate use.
