Accumulation of the amyloid-B peptide （AB） in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The B-amyloid protein precursor （APP） is cleaved by B-secretase，producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment （C99）. The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-B peptide，which is deposited in the brains of all suffers of Alzheimer’s disease.
應用類型
Western blot analysis （1-4 ug/ml） and Immunohistochemistry （2-10 ug/ml）. However， the optimal conditions should be determined individually. Murine and rat brain tissues can be used as positive controls.
免疫原
Synthetic peptide from human APP
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的APP蛋白發生免疫反應.
保存建議
提供的APP抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該APP抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
