The protein coding region of the catalytic domain of PTP-1B (amino acids 1-321) was cloned into an E. coli expression vector. The catalytic domain of PTP-1B was overexpressed in E. coli as a soluble protein, and it was purified by conventional column chromatographic techniques.
序列
重組人PTP1B 蛋白產品,又稱Protein tyrosine phosphatase, non-receptor type 1, PTPN1, Protein-tyrosine phosphatase 1B, Human Recombinant PTP1B,.其對應NCBI基因號為5770,對應Accession號為P18031.
來源宿主
提供的【PTP1B 】重組人PTP1B 產品的來源為大腸桿菌重組表達.
溶解建議
重組人PTP1B 產品為液體即用型,其溶液組成為:1 mg/ml solution in 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 2 mM β mercaptoethanol, 1 mM EDTA, 20% Glycerol.