CD73 （also designated ecto-5’-nucleotidase，E5NT，NT，NT5，NTE，eN and eNT） is a glycosyl-phosphatidylinositol （GPI）-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins，CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants，yet elicits diverse biological function through differential regulation in endothelial cells （EC），subpopulations of B and T cells，germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example，IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC，and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore，IgG-mediated targeting of lymphocyte CD73，but not of endothelial cell CD73，causes shedding of CD73 and tyrosine phosphorylation of proteins.
應用類型
WB: 1:1000， IHC: 1:10 – 1:50.
免疫原
This CD73 （NT5E） antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 （NT5E）.
