5-hydroxymethylcytosine （5-hmC） has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially，the 5-hmC bases have been identified in Purkinje neurons，in granule cells and embryonic stem cells where they are present at high levels （up to 0.6% of total nucleotides in Purkinje cells）. A recent report indicates that 5-hmC is abundant in brain tissue，especially in areas that are associated with higher cognitive functions. Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown，early evidence suggests a few putative mechanisms that could have big implications in epigenetics: 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and，as such open up entirely new perspectives in epigenetic studies Due to the structural similarity between 5-mC and 5-hmC，these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches （e.g. enzymatic approaches，bisulfite sequencing） do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique rabbit polyclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.
應用類型
hMeDIP: 2.5 ug/IP， Dot Blot: 1:200， ELISA: 1:500.
免疫原
Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH.
