中文名稱

【NEK6】NEK6蛋白，有活性的（5 ug）

英文名字

NEK6, Active

供應商

Biovision

產品貨號

7716-5

產品報價

￥已停產

產品說明書

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購買方式

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產品新聞

背景資料

Nek6 is a serine/threonine kinase that is a member of the Nek family of protein kinases that share an amino-terminal catalytic domain related to NIMA (never in mitosis, gene A) family (1). Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. Nek6 is phosphorylated and activated during M phase of the cell cycle (2). Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis suggesting that Nek6 is required for metaphase-anaphase transition. Nercc1/Nek9 binds to Nek6 and is likely to be responsible for the activation of Nek6 during mitosis representing a new signaling pathway that regulates mitotic progression (3). Northern blot analysis shows that Nek6 transcript is ubiquitously expressed with the highest expression found in the heart and skeletal muscle. Nek6 effectively phosphorylates histones H1 and H3, but not casein suggesting that, unlike other mammalian NIMA-related kinases, Nek6 is a mitotic histone kinase which regulates chromatin condensation in mammalian cells. In addition, Nek6 phosphorylates p70 S6 kinase at Thr412 and other sites and activates the p70 S6 kinase in vitro and in vivo, in a manner synergistic with PDK1 (4). Kinase-inactive Nek6 interferes with insulin activation of p70 S6 kinase implicating Nek6 as a possible physiologic regulator of the p70 S6 kinase.

序列

NEK6蛋白,有活性的蛋白產品，又稱NEK, Serine/threonine-protein kinase Nek6, Serine/threonine-protein kinase Nek6, Never in mitosis A-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512.其對應NCBI基因號為4067，NCBI基因別稱為Lyn B源，對應Accession號為BC059394.該蛋白產品又稱neb6, neb-6, neb 6, Active NEK6, NEK6, human NEK6, active kinase, active kinases, NEK, recombinant NEK6, recombinant NEK, kinase activity assay, protein kinase 等.

來源宿主

提供的【NEK6】NEK6蛋白,有活性的產品的來源為Sf9昆蟲細胞-桿狀病毒中重組表達.

溶解建議

提供的NEK6蛋白,有活性的 產品為液態形式溶解于儲存液(50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% 甘油)中.

保存建議

在您收到艾美捷發出的【NEK6】NEK6蛋白,有活性的產品后，建議將其置于-80°C保存.

其他

BioVision的公司總部在美麗的舊金山灣地區，它是全球生命科學公司的Leader，BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因子和酶等多種產品，以及其他創新的研究工具.其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉導、干細胞研究等領域.作為多家知名品牌的中國總代理以及中國區域代理，艾美捷與BioVision一起為研究者們提供優質、新的細胞生物學研究工具而不斷擴大產品及服務質量.

注意

該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。

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