中文名稱

【p70 S6K】p70 S6K蛋白，有活性的（5 ug）

英文名字

p70 S6K, Active

供應商

Biovision

產品貨號

7725-5

產品報價

￥已停產

產品說明書

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購買方式

銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868.

產品新聞

背景資料

Activation of cell growth leads to the multiple phosphorylation of 40S ribosomal protein S6. The kinase responsible for controlling this event is termed p70S6K (1). Northern blot analysis shows p70S6K to be ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation, and the serum-induced activation is inhibited by wortmannin and rapamycin. p70S6k activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0 (2). The kinase is reactivated 10-fold when cells released from a nocodazole-induced metaphase block enter G1 of the next cell cycle. The immunosuppressive agent rapamycin induces inactivation of p70s6k with no effect on other mitogen-activated kinases (3). The principal target of rapamycin-induced p70s6k inactivation is T389 site on p70S6K, which is located in an unusual hydrophobic sequence outside the catalytic domain. Mutation of T389 to alanine ablates kinase activity, whereas mutation to glutamic acid confers constitutive kinase activity and rapamycin resistance. p70S6K activation by growth factor requires phosphorylation by various inputs on multiple sites (4). p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as threonine 389. Threonine 229, a site in the catalytic loop is phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1). Activation of p70S6K requires a phosphoinositide 3-kinase (PI3-K)-dependent signal(s).

序列

p70 S6K蛋白,有活性的蛋白產品，又稱p70, Ribosomal protein S6 kinase beta-1, Serine/threonine-protein kinase 14A.其對應NCBI基因號為2268，NCBI基因別稱為FGR源，對應Accession號為P09769.該蛋白產品又稱p70s6k, p70-s6k, Active p70 S6K, p70 S6K, human p70 S6K, active kinase, active kinases, recombinant p70 S6K, recombinant p70 S6K, kinase activity assay, protein kinase等.

來源宿主

提供的【p70 S6K】p70 S6K蛋白,有活性的產品的來源為Sf9昆蟲細胞-桿狀病毒中重組表達.

溶解建議

提供的p70 S6K蛋白,有活性的 產品為液態形式溶解于儲存液(50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% 甘油)中.

保存建議

在您收到艾美捷發出的【p70 S6K】p70 S6K蛋白,有活性的產品后，建議將其置于-80°C保存.

其他

BioVision的公司總部在美麗的舊金山灣地區，它是全球生命科學公司的Leader，BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因子和酶等多種產品，以及其他創新的研究工具.其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉導、干細胞研究等領域.作為多家知名品牌的中國總代理以及中國區域代理，艾美捷與BioVision一起為研究者們提供優質、新的細胞生物學研究工具而不斷擴大產品及服務質量.

注意

該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。

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