【Pro MMP2】重組小鼠前基質金屬蛋白酶2（5 ug）-Pro-MMP-2, mouse代理/參數/廠家-重組蛋白-艾美捷科技有限公司

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背景資料: The progelatinase A, a member of the matrix metalloproteinase (MMP) family, has been isolated from macrophages and fibroblasts. Gelatinase A hydrolyses several components of the extracellular matrix, e.g. the collagen types IV, V and XI and gelatin. Progelatinase A complexed via their C-terminal domain with TIMP-2 was isolated from culture media of different cell types. This complex shows both properties of its constituents: Like TIMP-2 it inhibits active matrix metalloproteinases and like gelatinase it shows proteolytic activity after activation with APMA (4-aminophenylmercury acetate). However, its proteolytic activity is less than 10% of that of gelatinase A not complexed with TIMP-2. In contrast to the other MMPs the progelatinase A cannot be activated by the serine proteinase trypsin. Until quite recently a potential natural activator that can transform latent progelatinase A into the active form was unknown. It was shown that the catalytic domain of the membrane type 2-matrix metalloproteinase activates progelatinase A as well as the progelatinase A / TIMP-2 complex, by cleaving the 72 kDa progelatinase A to yield 67 kDa gelatinase A, which is than transformed into 62 kDa gelatinase A. The 62 kDa form is about twice as active as the 67 kDa form towards the Dnp-pepitde (Masui et al.). No significant difference in activity was found between free and complexed gelatinase A forms.

序列: 重組小鼠前基質金屬蛋白酶2蛋白產品，又稱72 kDa type IV collagenase (EC 3.4.24.24) (72 kDa gelatinase) (Gelatinase A) (Matrix metalloproteinase-2) (MMP-2) [Cleaved into: PEX].其對應NCBI基因號為17390，對應Accession號為Q3UG07.

溶解建議: 重組小鼠前基質金屬蛋白酶2產品為液體即用型，其溶液組成為：In 50 mM Tris-HCl, pH 7; 200 mM NaCl; 5 mM CaCl?; 1 uM ZnCl?; 0.05% Brij 35; 0,05% NaN?

其他: BioVision的公司總部在美麗的舊金山灣地區，它是全球生命科學公司的Leader，BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因子和酶等多種產品，以及其他創新的研究工具.其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉導、干細胞研究等領域.作為多家知名品牌的中國總代理以及中國區域代理，艾美捷與BioVision一起為研究者們提供優質、新的細胞生物學研究工具而不斷擴大產品及服務質量.