BioVision’s EZCut? TEV Protease is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). It contains an enhanced form of a catalytic fragment of the NIa protein of Tobacco etch virus (TEV). TEV Protease is a restriction grade protease that has robust activity at 4?C with high specificity and great stability. The optimal temperature for cleavage with this enzyme is 34°C. The protease can be used for the removal of affinity tags from fusion proteins. It contains a C-terminal His tag and can be easily removed after cleavage reactions by passing the reaction through a Ni-chelating resin. BioVision’s EZCut? TEV Protease is an improved version of TEV protease that is highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in enhanced long-term activity.
序列
提供的EZCut?TEV蛋白酶,重組EZCut? TEV Protease, Recombinant是Biovision品牌中國區域總代理艾美捷科技產品,具體的蛋白序列可查看提供的英文版本產品說明書。
來源宿主
提供的EZCut?TEV蛋白酶,重組EZCut? TEV Protease, Recombinant產品來源宿主,查看該產品說明書,或者在線咨詢我們。
溶解建議
該EZCut?TEV蛋白酶,重組EZCut? TEV Protease, Recombinant產品的溶解建議詳情請查閱產品說明書。