- 中文名稱
Antioxidant Assay Kit, BioAssay
- 英文名字
- Antioxidant Assay Kit, BioAssay
- 供應商
- USBiological
- 產品貨號
- A2298-44G
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- An Antioxidant is a molecule capable of slowing or preventing the oxidation of other molecules. Antioxidants protect the cells from damages by reactive oxygen species which are produced in oxidation reactions in the cell. Antioxidants can be small molecules such as glutathione, vitamins, or macromolecules such as catalase, glutathione peroxidase. As oxidative stress contributes to the development of many diseases including Alzheimer's disease, Parkinson's disease, diabetes, rheumatoid arthritis and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. Antioxidants are also widely used as dietary supplements and in industry as preservatives in food, cosmetics, rubber and gasoline.Simple, direct and high-throughput assays for total antioxidant capacity (TAC) find wide applications in research, food industry and drug discovery. The improved assay measures total antioxidant capacity in which Cu2+ is reduced by antioxidant to Cu+. The resulting Cu+ specifically forms a colored complex with a dye reagent. The color intensity at 570nm is proportional to TAC in the sample.Key Features:Sensitive and accurate. Use 20ul sample. Linear detection range from 1.5 to 1000uM Trolox equivalents.Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay for thousands of samples per day.Applications:Direct Assays: serum, plasma, urine, saliva and other biological samples, food and beverages.Drug Discovery/Pharmacology: effects of drugs on TAC.Kit Contents:Reagent A: 12ml Reagent B: 1mlStandard: 100ul 50mM TroloxStorage conditions. The kit is shipped at room temperature. Store Reagent A at room temperature and other components at -20 °C. Shelf life of six months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Sample Preparation:Samples should not contain any metal chelators (e.g. EDTA) and should be clear and free of any turbidity or particles. Liquid samples (e.g. nonhemolyzed serum, plasma) can be assayed directly. Cell lysate is prepared by homogenizing or sonicating cells in ice-cold 1 x PBS and centrifugation for 10 min at 14,000 rpm to pellet any debris. Use the clear supernatant for the assay. If not assayed immediately, freeze supernatant at -80°C (stable for 1 month).Assay Procedure:1. Standards and Samples. Equilibrate all components to room temperature. Briefly centrifuge Reagent B and Standard before opening. Mix 5ul of the standard with 245ul dH2O (final 1mM Trolox). Dilute standards as shown in the Table below. Transfer 20ul standards into wells of a clear flat-bottom 96-well plate.No 1mM Trolox+H2O Vol (ul) Trolox (uM)1 100ul+0ul 100 10002 60ul+40ul 100 6003 30ul+70ul 100 3004 0ul+100ul 100 0Transfer 20ul of each sample into separate wells of the 96-well plate.Note: for unknown samples, perform several dilutions to ensure that TAC is within the linear range of 1.5 to 1000uM Trolox equivalents.2. Assay. Prepare enough Working Reagent for Sample and Standard wells by mixing, for each assay well, 100ul Reagent A and 8ul Reagent B. Add 100ul Working Reagent to all assay wells. Tap plate to mix. Incubate 10 min at room temperature.3. Read OD570nm on a plate reader.Note: if calculated TAC is higher than 1000uM Trolox equivalents, dilute sample in dH2O and repeat assay. Multiply the results by the dilution factor.Calculation:Subtract blank OD value (#4) from all standard and sample OD values. Plot the DOD570nm against standard concentrations and determine the slope of the standard curve. Calculate the Total Antioxidant Capacity (TAC) of Sample,TAC =ODSAMPLE-ODBLANKSlope (uM-1)x n (uM Trolox Equivalents)ODSAMPLE and ODBLANK are the OD570nm values of the sample and H2O blank (standard #4). n is the sample dilution factor.Materials Required, But Not Provided:Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, plate reader capable of reading optical density at 570nm, homogenizer or sonicator etc.
- 產品特點
- 針對Antioxidant Assay Kit, BioAssay該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Antioxidant Assay Kit, BioAssay產品后將其置于RT/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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