Adenosine 5'-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery.Key Features:Safe. Non-radioactive assay.Sensitive and accurate. As low as 0.02uM ATP or a single cell can be quantified.Homogeneous and convenient. Mix-incubate-measure type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS: Z’ factors of > 0.5 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.Applications:ATP determination in cells and other biological samples.Kit Components:A3999-20A: Assay Buffer: 10mlA3999-20B: Substrate: 120ulA3999-20C: ATP Enzyme: 120ulA3999-20D: Standard, 3mM ATP: 100ulStorage and Stability:Store all reagents at -20°C. This kit is shipped on dry ice. Shelf life of the kit is 6 months after receipt.Precautions:Reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.General Considerations:Signal Stability: Since the signal of the reaction decreases by ~1% each minute, for most accurate results, care should be taken that the time between adding the Reconstituted Reagent and luminescence reading is the same for all samples and standards.
