Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Histidine--tRNA ligase cytoplasmic encoded by HARS is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for HARS. 蛋白別名為：HARS; HRS; Histidine--tRNA ligase; cytoplasmic; Histidyl-tRNA synthetase; HisRS;基因ID為：3035；蛋白質ID:P12081
應用類型
WB，IHC-p，IF，ELISA補充：最優的抗體稀釋比例需要基于客戶實驗進行優化.建議的起始稀釋比例如下: WB: 1:500-1:2000, IHC-p: 1:100-1:300, IF: 1:200-1:1000, ELISA: 1:20000. Not yet tested in other applications.
免疫原
合成多肽：the C-terminal region of human HisRS. at AA rangle: 430-510
