中文名稱

JOE phosphoramidite, 6-isomer

英文名字

JOE phosphoramidite, 6-isomer

供應商

antibodies

產品貨號

ANT-A270233-5

產品報價

￥詢價/5g

產品說明書

點擊查看

購買方式

銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系。本公司所有產品僅供科研使用，不用于臨床診斷。

產品新聞

背景資料

該JOE phosphoramidite, 6-isomer的背景資料請參考antibodies.com的說明書

產品描述

JOE phosphoramidite for oligonucleotide synthesis, pure 6-isomer (6-JOE). Fluorescent dye JOE is a fluorescein derivative containing two chlorine atoms and two methoxy groups. Its absorption and emission maxima are at 503 nm and 525 nm, respectively. By its spectral characteristics JOE is found in between FAM and TAMRA/ROX; therefore, this fluorophor is commonly used for multiplex detection, including that during DNA sequencing. Coupling time of 6 minutes. Deprotection can be perfromed under standard conditions using ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 h at 55 °C removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% of non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (e.g. 10 min at 65 °C).

產品形式

保存建議

該JOE phosphoramidite, 6-isomer的保存建議請參考antibodies.com的說明書

其他

antibodies.com 以具有成本效益的價格向生命科學研究人員提供高質量的生物試劑。公司總部位于劍橋（英國），并在斯德哥爾摩（瑞典）和圣路易斯（美國密蘇里州）設有辦事處，可提供包括一抗、二抗、蛋白質和肽、免疫分析和試劑盒以及其他相關產品。

注意

該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。

分享到：