中文名稱

TAMRA phosphoramidite, 5-isomer

英文名字

TAMRA phosphoramidite, 5-isomer

供應商

antibodies

產品貨號

ANT-A270325-1

產品報價

￥詢價/1g

產品說明書

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購買方式

銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系。本公司所有產品僅供科研使用，不用于臨床診斷。

產品新聞

背景資料

該TAMRA phosphoramidite, 5-isomer的背景資料請參考antibodies.com的說明書

產品描述

This phosphoramidite is used for synthesis of oligonucleotides 5'-labeled with TAMRA. TAMRA (carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orange spectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor (and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of their spectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, which contain 5'-terminal TAMRA and FAM in the middle of the sequence or at the 3'-end (using Fluorescein dT Phosphoramidite and FAM CPG, respectively). TAMRA 5'-labeled oligonucleotides are commonly used for quantitative PCR and fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detection channel for TAMRA frequently. The TAMRA dye is not stable in the presence of ammonium and sterically non-hindered primary amines, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection. Coupling takes only 7.5 minutes. Deprotection should be performed with tret-buthylamine : methanol : water at a ratio of 1 : 1 : 3 (v/v/v) ("TAMRA cocktail") for 6 hours at 60 °C, then cooled down to room temperature. Due to complete and irreversible degradation of the TAMRA dye, do NOT use aqueous ammonium and AMA for deprotecting a modified oligonucleotide from the solid-phase support.

產品形式

保存建議

Shipped at room temperature. Upon delivery, store in the dark at -20

其他

antibodies.com 以具有成本效益的價格向生命科學研究人員提供高質量的生物試劑。公司總部位于劍橋（英國），并在斯德哥爾摩（瑞典）和圣路易斯（美國密蘇里州）設有辦事處，可提供包括一抗、二抗、蛋白質和肽、免疫分析和試劑盒以及其他相關產品。

注意

該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。

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