山羊抗小鼠IgG1（亞類特異性），HRP偶聯-Goat anti-Mouse IgG1 (subclass specific), HRP conjugated代理/參數/廠家-植物抗體-艾美捷科技有限公司

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背景資料: The reactivity of the antiserum is directed to the subclass IgG1. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG1 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG1 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG1 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG1 in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1.000 and 1:10,000 depending on the method used.

應用類型: Dot blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)

免疫原: Pools of purified homogenous IgG1 isolated from pooled mouse serum, Freund's complete adjuvant is used in the first step of the immunization procedure,

保存建議: The lyophilized conjugate is shipped at ambient temperature and may be stored at 4

其他: Agrisera公司提供1000種用于植物和藻類細胞生物學研究的一抗，2000多種二抗。Agrisera公司提供的植物領域的抗體涵蓋擬南芥、萊茵衣藻、藍藻、松柏類、硅藻、大豆、大麥、蒺藜苜蓿、煙草、稻、菜豆、小立碗蘚、楊樹、豌豆、番茄、馬鈴薯、菠菜、小麥、玉米、葡萄等多個物種，此外還有水生生物類（藻類、魚）抗體，細菌、昆蟲、真菌抗體。針對植物蛋白質的保守氨基酸序列，Agirsera還獨家開發了數十種通用抗體（global antibodies），這類抗體幾乎可以與所有的高等植物及藻類產生免疫反應，可為研究人員提供極為方便的選擇。