- 中文名稱
Bromodeoxyuridine (BrdU) Cell Proliferation Kit, BioAssay
- 英文名字
- Bromodeoxyuridine (BrdU) Cell Proliferation Kit, BioAssay
- 供應商
- USBiological
- 產品貨號
- B2851
- 產品報價
- ￥1/50 Slides
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- In the past, most cell-proliferation studies have used radioactive thymidine as an incorporated label to give evidence of DNA replication. Recently, a less hazardous technique has been developed using bromodeoxyuridine (BrdU), a thymidine analog, in lieu of a radioactive reagent. (1) BrdU is incorporated into proliferating cells (S-phase) much in the same way as radioactive thymidine, but is then detected by a monoclonal anti-BrdU antibody, and revealed using a highly sensitive streptavidin- biotin staining system. BrdU has proven useful for proliferative studies of normal and neoplastic tissues both in vivo and in vitro.The BrdU staining system uses a biotinylated monoclonal anti-BrdU, thus eliminating the need for a species-specific secondary antibody. As a result, BrdU labeling can be performed in rats and mice without problems of crossreactivity or spurious staining.Streptavidin-peroxidase is used as a signal generator for the BrdU system. Diaminobenzidine (DAB) in the presence of hydrogen peroxide is used as a chromogen, staining BrdU-incorporated nuclei dark brown. This kit also contains four BrdU control slides for the convenience of the investigator: four unstained positive controls.Kit Components:B2851-01 Trypsin Concentrate 1x3mlB2851-02 Trypsin Diluent 1x12mlB2851-03 Denaturing Solution 1x6ml (Ready-to-use) B2851-04 Blocking Solution 1x6ml(Ready-to-use)B2851-05 BrdU (Biotin) 1x6ml (Ready-to-use) B2851-06 Streptavidin (HRP) 1x6ml (Ready-to-use)B2851-07 Substrate Buffer, 20x 1x2mlB2851-08 DAB, 20x 1x2mlB2851-09 0.6% Hydrogen Peroxide, 20x 1x2mlB2851-10 Hematoxylin 1x6ml (Ready-to-use)B2851-11 Histomount 1x6ml (Ready-to-use) B2851-12 Control Slides 4x Unstained Controls Materials Required but not Provided:-Phosphate Buffered Saline (PBS)-Quenching solution for endogenous peroxidase-Alcohol and xylene-Cover slipsStorage and Stability:Store at 4°C. Stable for at least 6 months.
- 產品特點
- 針對Bromodeoxyuridine (BrdU) Cell Proliferation Kit, BioAssay該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Bromodeoxyuridine (BrdU) Cell Proliferation Kit, BioAssay產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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