The mouse CD30 ligand (CD30L) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse CD30L antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known CD30L concentration are added to these wells and allowed to complex with the bound CD30L antibody. A biotinylated mouse CD30L polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD30L present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CD30L in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
產品特點
靈敏度:15.6 pg/mL - 1000 pg/mL。此外該ELIAS試劑盒包含：2 x 96孔預包被條孔板（可拆卸）, 蛋白質標準品，檢測試劑，底物緩沖液，以及詳細的實驗方案。
