L-selection (CD26L) is a cell surface adhesion protein constitutively expressed on all classes of human leukocytes. The L-Selectin glycoprotein is composed of a C-type lectin family domain at the amino terminus, an EGF-like domain, two short consensus repeat (SCR) sequences, a transmembrane domain and a short cytoplasmic tail. L-Selectin contains 7 potential N- linked glycosylation sites.
產品描述
The mouse L-Selectin Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse L-Selectin antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known L-Selectin concentration are added to these wells and allowed to complex with the bound L-Selectin antibody. A biotinylated mouse monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of L-Selectin present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse L-Selectin in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
產品特點
靈敏度:62.5 pg/mL - 4000 pg/mL。此外該ELIAS試劑盒包含：1 x 96孔預包被條孔板（可拆卸）, 蛋白質標準品，檢測試劑，底物緩沖液，以及詳細的實驗方案。
