Cy5 DBCO NHS Ester is a bright, far red fluorescent dual-label click chemistry probe that allows for simultaneous attachment of DBCO moiety and far red fluorescent Cy5 label. Conjugates prepared with Cy5 DBCO NHS Ester reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Cy5 DBCO is an ideal alternative to copper requiring fluorescent alkynes.nnCy5 dye is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. Its absorption and emission spactra are almost identical to those of Alexa Fluor? 647, CF? 647 Dye, or any other Cyanine5 based fluorescent dyes.
