Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”.nnCy3 is a water-soluble, red fluorescent pH-insensitive from pH 4 to pH 10 probe. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor? 555, CF? 555 Dye or any other Cyanine3 based fluorescent dyes.
