BioVision's Prostaglandin D2 ELISA kit is based on Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGD2. During the reaction, PGD2 in the sample or standard competes with a fixed amount of PGD2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGD2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PGD2 in the samples is then determined by comparing the OD of the samples to the standard curve.
