PPZ-A10 is an ionizable lipid used for formulating lipid nanoparticles to deliver RNA in vitro and in vivo. PPZ-A10 LNPs preferentially delivered Cre mRNA to liver and spleen immune cells when injected intravenously into mice at concentrations as low as 0.3 mg/kg. PPZ-A10 LNPs containing RNA to generate chimeric antigen receptor macrophages were injected into a hepatocellular carcinoma mouse model resulting in decreased tumor burden and increased survival.
