This ELISA Kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat COL1α1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with?the specific antibody. Then a biotinylated detection antibody specific for Rat COL1α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain?Rat COL1α1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns?yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of?Rat COL1α1.?The concentration of?Rat COL1α1?in samples can be calculated by comparing the OD of the samples to the standard curve.
產品特點
Colorimetric
保存建議
Biomatik品牌廠家建議該大鼠COL1α1(CollagenTypeⅠAlpha1)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
