This ELISA Kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat PGF2α. During the reaction, Rat PGF2α in the sample or standard competes with a fixed amount of?Rat PGF2α?on the solid phase supporter?for sites on the Biotinylated Detection Ab specific to?Rat PGF2α. Excess conjugate and unbound sample or standard are washed from the plate, and?Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then?a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of??Rat PGF2α?in the samples is then determined by comparing the OD of the samples to the standard curve.
產品特點
Colorimetric
保存建議
Biomatik品牌廠家建議該大鼠PGF2α（前列腺素F2Alpha）ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
